Fluorescence Filter Sets
AHF analysentechnik offers a wide variety of single band fluorescence filter sets which have been optimized for use in a variety of fluorescence instruments. All sets are thin-film, hard-coated filters for excellent transmission performance and durability. Each filter set consists of individual excitation, dichroic, and emission filters. Please click on a filter set to access detailed individual filter specifications. The excitation filter will select the wavelength to excite the fluorophore, the emission filter determines the spectral window where the emitted light will be detected. The dichroic must reflect the excitation light and transmit the emitted light. The filters in these sets are sized to be compatible with many microscopes, including the Olympus AX, BX and IX series, microscopes cubes U-MF2/U-FF and Nikon microscopes E200, E400, E600, E800, E1000, TS100, TS100F, TE200, TE300, as well they fit into Nikon microscopes TE2000, 50i, 55i, 80i, 90i, Eclipse Ti, with the TE2000 cube.
Filter cubes with special-sized filters (non-standard)
All these filtersets can be be adapted to old fluorescence microscope types like JENAMED or JENALUMAR. Nikon Diaphot, Olympus CK40 or Olympus BH2 and to actual stereo microscope models like Stereo Discovery or Stereo Lumar V.12, or stereo models from Nikon like SMZ 1270, SMZ 1000/1500 or SMZ 18/25. For Leica microscopes (DM, DMI, DMR, DMLB, DMIR, DMRXA, DMRBE, DMLS) all listed filter sets can be mounted into the DM”K” or DM”L” cubes, for older models into DM”S” cubes. Leica stereo microscope models like MZ FL III or MZ 16FA require special holders which can be offered as well. Also filter cubes for Motic microscope models or Keyence are available. For non-standard applications like TIRF, special TIRF cubes must be asked for.
Complete filter sets for fluorophores
The spectral range of the filter sets starts from deep UV-filter sets to blue, green, yellow, orange, red and NIR. They are precisely matched to fluorescent dyes resp. fluorophores and fluorescence excitation light sources. To achieve best signal/noise ratios selective or narrowband filter sets are recommended. Longpass filter sets can be helpful in case of very week signals or uncertain spectral emission signals. Filter sets for blue, green, yellow and red fluorescent dyes are used in many immunofluorescent applications. For DNA or RNA Hybridisation analysis, many special filter sets (mFISH series) are offered. For multicolor applications, extremely narrowband filters are requested. Dualband versions like green/orange or green/red or tripleband filters blue/green/orange-red are very often used in FISH laboratories for visual control of DNA samples in prenatal diagnostic or forensics.