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Optical Filters for TIRF Applications

AHF analysentechnik offers you carefully selected optical filters for TIRF applications, which are mounted in special TIRF filter cubes. They are available for microscopes from Nikon, Olympus, Zeiss and Leica. Various optical singleband and multiband filter sets are available, mounted into microscope filter cubes. Customer-specific solutions are also possible. As a special service, AHF also provides filter components for testing with your microscope.

AHF analysentechnik offers you carefully selected optical filters for TIRF applications, which are mounted in special TIRF filter cubes. They are available for microscopes from Nikon, Olympus,... read more »
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Optical Filters for TIRF Applications

AHF analysentechnik offers you carefully selected optical filters for TIRF applications, which are mounted in special TIRF filter cubes. They are available for microscopes from Nikon, Olympus, Zeiss and Leica. Various optical singleband and multiband filter sets are available, mounted into microscope filter cubes. Customer-specific solutions are also possible. As a special service, AHF also provides filter components for testing with your microscope.

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No results were found for the filter!
Price on request
AOI 45° Reflection 488, 561 nm >90% A/R Coating lambda/10 substrate Dimension 36 x 25,5 x 2 mm
Content: 1 Pieces
€576.00 *
AOI 45° Reflection 532, 635-640 nm >90% Transmission 550-615 and 660-750 nm >90% A/R coating Dimension 25,5 x36 x 2 mm
Content: 1 Pieces
€576.00 *
AOI 0° A/R Coating Diameter 25 mm
Content: 1 Pieces
€866.00 *
AOI 45° Reflection 350-442 nm >90% Transmission 460 - 650 nm >90% A/R coating Dimension 25,5 x 36 x 2 mm
Content: 1 Pieces
€425.00 *
filter mounting included for beamsplitters up to 3 mm thickness
Content: 1 Pieces
€425.00 *
filter mounting included
Content: 1 Pieces
€417.00 *
TIRF filtermodule compatibel for Nikon i-Serie/TE2000-Mikroscopes Please note: This part is only available with Chroma-beamsplitters! filter mounting included
Price on request
TIRF filtermodule compatibel for Olympus BX2/IX2-Mikroscopes for use of up to 3 mm thick (TIRF-)beamsplitters Please note: This part is only available with Chroma-beamsplitters! incl. mounting of filters
Content: 1 Pieces
€760.00 *
TIRF filtermodule compatibel for Olympus BX3/IX3-Mikroscopes for use of 2 mm thick (TIRF-)beamsplitters Please note: This part is only available with Chroma-beamsplitters! incl. mounting and adjusting of filters
Content: 1 Pieces
€760.00 *
AOI 45° Reflection 405, 488, 532, 640 nm >90% A/R Coating RWD 0,5 lambda PV/inch Dimension 25,5 x 36 x 2 mm
Content: 1 Pieces
€794.00 *
AOI 45° Reflection 405, 488, 561,633-640 nm >90% A/R Coating for TIRF Dimension 36 x 25,5 x 2 mm
Content: 1 Pieces
€667.00 *
AOI 45° Reflection 405, 488, 561, 647 nm >90% A/R Coating for TIRF Dimension 36 x 25,5 x 2 mm
Content: 1 Pieces
€688.00 *
AOI 45° Reflection 488, 543,635, 730 nm >90% A/R Coating lambda/10 substrate Dimension 36 x 25.5 x 2 mm
Content: 1 Pieces
€919.00 *
AOI 0° diameter 25 mm
Content: 1 Pieces
€957.00 *
AOI 0° diameter 25 mm
Content: 1 Pieces
€957.00 *
Andere Bandpässe möglich. Bitte nehmen Sie Kontakt mit uns auf!
Price on request
consists of: Exciter: 390/40 (hartcoated); Beamsplitter: Reflection 380-405 nm >90%, Transmission 420 - 800 nm >90% (hartcoated); Emitter: 460/50 (hartcoated); mounted in TIRF-compatible OLYMPUS filter cube for BX2/IX2 Other combinations...
Price on request
consists of: Exciter: 390/40 (hartcoated); Beamsplitter: Reflection 380-405 nm >90%, Transmission 420 - 800 nm >90% (hartcoated); Emitter: 460/50 (hartcoated); mounted in TIRF-compatible OLYMPUS filter cube for BX2/IX2 Other combinations...
Price on request
consists of: Exciter: 390/40 (hartcoated); Beamsplitter: Reflection 380-405 nm >90%, Transmission 420 - 800 nm >90% (hartcoated); Emitter: 460/50 (hartcoated); mounted in TIRF-compatible ZEISS filter cube Other combinations possible....
Price on request
consists of: Exciter: 438/24 (hartcoated); Beamsplitter: Reflection 420-445 nm >90%, Transmission 460 - 800 nm >90% (hartcoated); Emitter: 483/32 (hartcoated); mounted in TIRF-compatible NIKON filter cube Other combinations possible....
Price on request
consists of: Exciter: 438/24 (hartcoated); Beamsplitter: Reflection 420-445 nm >90%, Transmission 460 - 800 nm >90% (hartcoated); Emitter: 483/32 (hartcoated); mounted in TIRF-compatible OLYMPUS filter cube for BX2/IX2 Other combinations...
Price on request
consists of: Exciter: 438/24 (hartcoated); Beamsplitter: Reflection 420-445 nm >90%, Transmission 460 - 800 nm >90% (hartcoated); Emitter: 483/32 (hartcoated); mounted in TIRF-compatible OLYMPUS filter cube for BX2/IX2 Other combinations...
Price on request
consists of: Exciter: 438/24 (hartcoated); Beamsplitter: Reflection 420-445 nm >90%, Transmission 460 - 800 nm >90% (hartcoated); Emitter: 483/32 (hartcoated); mounted in TIRF-compatible ZEISS filter cube Other combinations possible....
Price on request
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TIRF Requires Good Filter Specification

Within wide field microscopy, TIRF (Total Internal Reflection Fluorescence) microscopy has developed as a routine method that allows a significant contrast enhancement of the sample to be analysed in the evanescent field. To achieve this TIRF mode, some technical requirements have to be met on the microscope. In addition to specific lasers and high-aperture objectives, the filter specification must be taken into account.

Suitable Beam Splitters For TIRF

Optimum examination results in TIRF microscopy require beam splitters of high optical quality, which must be installed stress-free to avoid distortion of the laser beam profile. These are combined with the respective clean-up filters and blocking filters. Depending on the application, up to four different laser wavelengths can be coupled simultaneously via a TIRF cube (TIRF quad-band cube). Special TIRF beam splitters can be mounted in TIRF-specific filter cubes without any stress, which greatly simplifies microscopic applications.

TIRF Filters From Renowned Manufacturers

In cooperation with renowned filter manufacturers such as Chroma Technology and Semrock (IDEX), we offer complete filter sets, which are specifically adapted to the requirements of the measuring station and the questions of the TIRF user and are elaborated with great care. We will be pleased to advise you, independent of the manufacturer, on the selection of the appropriate TIRF filter combination for your microscope. Please contact us!