TIRF Requires Good Filter Specification
Within wide field microscopy, TIRF (Total Internal Reflection Fluorescence) microscopy has developed as a routine method that allows a significant contrast enhancement of the sample to be analysed in the evanescent field. To achieve this TIRF mode, some technical requirements have to be met on the microscope. In addition to specific lasers and high-aperture objectives, the filter specification must be taken into account.
Suitable Beam Splitters For TIRF
Optimum examination results in TIRF microscopy require beam splitters of high optical quality, which must be installed stress-free to avoid distortion of the laser beam profile. These are combined with the respective clean-up filters and blocking filters. Depending on the application, up to four different laser wavelengths can be coupled simultaneously via a TIRF cube (TIRF quad-band cube). Special TIRF beam splitters can be mounted in TIRF-specific filter cubes without any stress, which greatly simplifies microscopic applications.
TIRF Filters From Renowned Manufacturers
In cooperation with renowned filter manufacturers such as Chroma Technology and Semrock (IDEX), we offer complete filter sets, which are specifically adapted to the requirements of the measuring station and the questions of the TIRF user and are elaborated with great care. We will be pleased to advise you, independent of the manufacturer, on the selection of the appropriate TIRF filter combination for your microscope. Please contact us!